› 4r71

X-ray diffraction
3.21Å resolution

Structure of the Qbeta holoenzyme complex in the P1211 crystal form

Released:
DOI: 10.2210/pdb4r71/pdb
PDB entry 4r71 coloured by chain, front view.
PDB entry 4r71 coloured by chain, side view.
PDB entry 4r71 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for RNA-genome recognition during bacteriophage Qβ replication.
Gytz H, Mohr D, Seweryn P, Yoshimura Y, Kutlubaeva Z, Dolman F, Chelchessa B, Chetverin AB, Mulder FA, Brodersen DE, Knudsen CR
Nucleic Acids Res 43 10893-906 (2015)
PMID: 26578560

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-141405 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Elongation factor Ts; Elongation factor Tu 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 694 amino acids
Theoretical weight: 75.39 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6P1 (Residues: 1-283; Coverage: 100%)
  • Canonical: P0CE48 (Residues: 1-394; Coverage: 100%)
Gene names: JW0165, JW3943, b0170, b3980, tsf, tufB
Sequence domains:
Structure domains:
RNA-directed RNA polymerase subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 595 amino acids
Theoretical weight: 66 KDa
Source organism: Qubevirus durum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14647 (Residues: 1-589; Coverage: 100%)
Sequence domains: RNA replicase, beta-chain
Small ribosomal subunit protein bS1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 171 amino acids
Theoretical weight: 18.8 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AG67 (Residues: 2-171; Coverage: 31%)
Gene names: JW0894, b0911, rpsA, ssyF
Sequence domains: S1 RNA binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 99.73Å b: 115.45Å c: 178.52Å
α: 90° β: 96.14° γ: 90°
R-values:
R R work R free
0.216 0.213 0.268
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

RNA Phage Biology in a Metagenomic Era.
Callanan et al. (2018)
1 more

6 mentions without citation

RNA Dependent RNA Polymerases: Insights from Structure, Function and Evolution.
Venkataraman et al. (2018)
5 more