› 4r7e

X-ray diffraction
2.25Å resolution

Structure of Bre1 RING domain

Released:
DOI: 10.2210/pdb4r7e/pdb
PDB entry 4r7e coloured by chain, front view.
PDB entry 4r7e coloured by chain, side view.
PDB entry 4r7e coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the yeast Bre1 RING domain.
Kumar P, Wolberger C
Proteins 83 1185-90 (2015)
PMID: 25864391

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170475 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase BRE1 Chain: A
Molecule details ›
Chain: A
Length: 69 amino acids
Theoretical weight: 7.89 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07457 (Residues: 632-700; Coverage: 10%)
Gene names: BRE1, YDL074C
Sequence domains: Zinc finger, C3HC4 type (RING finger)
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P6122
Unit cell:
a: 56.765Å b: 56.765Å c: 134.858Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.22 0.247
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Bre1/Rad6 machinery: writing the central histone ubiquitin mark on H2B and beyond.
Deng et al. (2020)
1 more

6 mentions without citation

Structural mechanism for the recognition and ubiquitination of a single nucleosome residue by Rad6-Bre1.
Gallego et al. (2016)
5 more