› 4r7q

X-ray diffraction
1.98Å resolution

The structure of a sensor domain of a histidine kinase from Vibrio cholerae O1 biovar eltor str. N16961

Released:
DOI: 10.2210/pdb4r7q/pdb
PDB entry 4r7q coloured by chain, front view.
PDB entry 4r7q coloured by chain, side view.
PDB entry 4r7q coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Primary publication:
Sensor Domain of Histidine Kinase VxrA of Vibrio cholerae- A Hairpin-swapped Dimer and its Conformational Change.
Tan K, Teschler JK, Wu R, Jedrzejczak RP, Zhou M, Shuvalova LA, Endres MJ, Welk LF, Kwon K, Anderson WF, Satchell KJF, Yildiz FH, Joachimiak A
J Bacteriol (2021)
PMID: 33753465

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensor histidine kinase VxrA Chain: A
Molecule details ›
Chain: A
Length: 222 amino acids
Theoretical weight: 25.07 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KM24 (Residues: 38-256; Coverage: 44%)
Gene names: VC_A0565, vxrA
Sequence domains: Domain of unknown function (DUF3404)

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 1 x GOL
Ligand ACT 1 x ACT
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 88.6Å b: 88.6Å c: 106.993Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Diversity in Sensing and Signaling of Bacterial Sensor Histidine Kinases.
Ishii et al. (2021)
2 other articles

2 mentions without citation

Protein structure prediction using residue- and fragment-environment potentials in CASP11.
Kim et al. (2016)
1 more