› 4r89

X-ray diffraction
4Å resolution

Crystal structure of paFAN1 - 5' flap DNA complex with Manganase

Released:
DOI: 10.2210/pdb4r89/pdb
PDB entry 4r89 coloured by chain, front view.
PDB entry 4r89 coloured by chain, side view.
PDB entry 4r89 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Crystal structure of a Fanconi anemia-associated nuclease homolog bound to 5' flap DNA: basis of interstrand cross-link repair by FAN1.
Gwon GH, Kim Y, Liu Y, Watson AT, Jo A, Etheridge TJ, Yuan F, Zhang Y, Kim Y, Carr AM, Cho Y
Genes Dev 28 2276-90 (2014)
PMID: 25319828

Function and Biology Details

Reaction catalysed: 
Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118081 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Fanconi-associated nuclease 1 homolog Chains: A, E
Molecule details ›
Chains: A, E
Length: 559 amino acids
Theoretical weight: 64.61 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I2N0 (Residues: 1-559; Coverage: 100%)
Gene names: PA1865, fan1
Sequence domains:
DNA (5'-D(P*AP*CP*CP*AP*GP*AP*CP*AP*CP*AP*CP*AP*TP*TP*C)-3') Chains: B, F
Molecule details ›
Chains: B, F
Length: 15 nucleotides
Theoretical weight: 4.51 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Not provided
DNA (5'-D(P*GP*TP*TP*GP*GP*GP*AP*TP*TP*G)-3') Chains: C, G
Molecule details ›
Chains: C, G
Length: 10 nucleotides
Theoretical weight: 3.13 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Not provided
DNA (5'-D(P*GP*AP*AP*TP*GP*TP*GP*TP*GP*TP*CP*TP*CP*AP*AP*TP*CP*CP*CP*AP*AP*C)-3') Chains: D, H
Molecule details ›
Chains: D, H
Length: 22 nucleotides
Theoretical weight: 6.71 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 81.115Å b: 106.944Å c: 107.541Å
α: 90° β: 89.84° γ: 90°
R-values:
R R work R free
0.226 0.224 0.278
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Fanconi anaemia and cancer: an intricate relationship.
Nalepa et al. (2018)
1 more

3 mentions without citation

Crystal structure of a Fanconi anemia-associated nuclease homolog bound to 5' flap DNA: basis of interstrand cross-link repair by FAN1.
Gwon et al. (2014)
2 more