› 4r8g

X-ray diffraction
3.5Å resolution

Crystal Structure of Myosin-1c tail in complex with Calmodulin

Released:
DOI: 10.2210/pdb4r8g/pdb
PDB entry 4r8g coloured by chain, front view.
PDB entry 4r8g coloured by chain, side view.
PDB entry 4r8g coloured by chain, top view.
Source organisms:
Primary publication:
Structure of myosin-1c tail bound to calmodulin provides insights into calcium-mediated conformational coupling.
Lu Q, Li J, Ye F, Zhang M
Nat Struct Mol Biol 22 81-8 (2015)
PMID: 25437912

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144052 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Unconventional myosin-Ic Chain: E
Molecule details ›
Chain: E
Length: 335 amino acids
Theoretical weight: 38.11 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WTI7 (Residues: 733-1063; Coverage: 31%)
Gene name: Myo1c
Sequence domains:
Calmodulin-1 Chains: A, B, H
Molecule details ›
Chains: A, B, H
Length: 148 amino acids
Theoretical weight: 16.72 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP33 (Residues: 2-149; Coverage: 99%)
Gene name: calm1
Sequence domains: EF-hand domain pair

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43212
Unit cell:
a: 105.447Å b: 105.447Å c: 288.068Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.242 0.304
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Cytoskeleton Molecular Motors: Structures and Their Functions in Neuron.
Xiao et al. (2016)
6 more

11 mentions without citation

Myosin light chains: Teaching old dogs new tricks.
Heissler et al. (2014)
10 more