› 4r8h

X-ray diffraction
1.46Å resolution

The role of protein-ligand contacts in allosteric regulation of the Escherichia coli Catabolite Activator Protein

Released:
DOI: 10.2210/pdb4r8h/pdb
PDB entry 4r8h coloured by chain, front view.
PDB entry 4r8h coloured by chain, side view.
PDB entry 4r8h coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The Role of Protein-Ligand Contacts in Allosteric Regulation of the Escherichia coli Catabolite Activator Protein.
Townsend PD, Rodgers TL, Glover LC, Korhonen HJ, Richards SA, Colwell LJ, Pohl E, Wilson MR, Hodgson DR, McLeish TC, Cann MJ
J Biol Chem 290 22225-35 (2015)
PMID: 26187469

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142283 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding transcriptional dual regulator CRP Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 25.08 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACJ8 (Residues: 1-210; Coverage: 100%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SP1 4 x SP1
Ligand GOL 7 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 45.73Å b: 102.26Å c: 54.3Å
α: 90° β: 111.55° γ: 90°
R-values:
R R work R free
0.189 0.187 0.215
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Signalling networks and dynamics of allosteric transitions in bacterial chaperonin GroEL: implications for iterative annealing of misfolded proteins.
Thirumalai et al. (2018)
2 more

5 mentions without citation

The Transcriptional Regulators of the CRP Family Regulate Different Essential Bacterial Functions and Can Be Inherited Vertically and Horizontally.
Soberón-Chávez et al. (2017)
4 more