› 4r8w

X-ray diffraction
2.8Å resolution

Crystal structure of H7 hemagglutinin from A/Anhui/1/2013 in complex with a neutralizing antibody CT149

Released:
DOI: 10.2210/pdb4r8w/pdb
PDB entry 4r8w coloured by chain, front view.
PDB entry 4r8w coloured by chain, side view.
PDB entry 4r8w coloured by chain, top view.
Source organisms:
Primary publication:
A potent broad-spectrum protective human monoclonal antibody crosslinking two haemagglutinin monomers of influenza A virus.
Wu Y, Cho M, Shore D, Song M, Choi J, Jiang T, Deng YQ, Bourgeois M, Almli L, Yang H, Chen LM, Shi Y, Qi J, Li A, Yi KS, Chang M, Bae JS, Lee H, Shin J, Stevens J, Hong S, Qin CF, Gao GF, Chang SJ, Donis RO
Nat Commun 6 7708 (2015)
PMID: 26196962

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-234881 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 34.99 KDa
Source organism: Influenza A virus (A/Anhui/1-BALF_RG45/2013(H7N9))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A0A024E3P0 (Residues: 19-339; Coverage: 57%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin Chain: B
Molecule details ›
Chain: B
Length: 177 amino acids
Theoretical weight: 20.44 KDa
Source organism: Influenza A virus (A/Anhui/1-BALF_RG45/2013(H7N9))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A0A024E2M1 (Residues: 340-516; Coverage: 32%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B
Heavy chain of neutralizing antibody CT149 Chain: H
Molecule details ›
Chain: H
Length: 231 amino acids
Theoretical weight: 24.43 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Structure domains: Immunoglobulins
Light chain of neutralizing antibody CT149 Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 23.76 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: R32
Unit cell:
a: 126.856Å b: 126.856Å c: 409.596Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.265 0.262 0.311
Expression system: Spodoptera frugiperda

Quick links

Citations

31 review citations

Beyond binding: antibody effector functions in infectious diseases.
Lu et al. (2018)
30 more

9 mentions without citation

Vaccine-Induced Antibodies that Neutralize Group 1 and Group 2 Influenza A Viruses.
Joyce et al. (2016)
8 more