› 4rav

X-ray diffraction
2.5Å resolution

Crystal structure of scFvC4 in complex with the first 17 AA of huntingtin

Released:
DOI: 10.2210/pdb4rav/pdb
PDB entry 4rav coloured by chain, front view.
PDB entry 4rav coloured by chain, side view.
PDB entry 4rav coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a single-chain Fv bound to the 17 N-terminal residues of huntingtin provides insights into pathogenic amyloid formation and suppression.
De Genst E, Chirgadze DY, Klein FA, Butler DC, Matak-Vinković D, Trottier Y, Huston JS, Messer A, Dobson CM
J Mol Biol 427 2166-78 (2015)
PMID: 25861763

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211210 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Single-chain Fv, VL Chains: B, D
Molecule details ›
Chains: B, D
Length: 117 amino acids
Theoretical weight: 12.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
single-chain Fv, VH Chains: A, C
Molecule details ›
Chains: A, C
Length: 126 amino acids
Theoretical weight: 13.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Huntingtin Chains: E, F
Molecule details ›
Chains: E, F
Length: 17 amino acids
Theoretical weight: 1.98 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P42858 (Residues: 1-17; Coverage: 1%)
Gene names: HD, HTT, IT15

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 151.313Å b: 35.933Å c: 110.946Å
α: 90° β: 120.72° γ: 90°
R-values:
R R work R free
0.182 0.18 0.226
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Protein Folding and Mechanisms of Proteostasis.
Díaz-Villanueva et al. (2015)
6 more

3 mentions without citation

Conformational selection in amyloid-based immunotherapy: Survey of crystal structures of antibody-amyloid complexes.
Ma et al. (2016)
2 more