› 4rb1

X-ray diffraction
2.75Å resolution

Crystal structure of Magnetospirillum gryphiswaldense MSR-1 Fur-Mn2+-E. coli Fur box

Released:
DOI: 10.2210/pdb4rb1/pdb
PDB entry 4rb1 coloured by chain, front view.
PDB entry 4rb1 coloured by chain, side view.
PDB entry 4rb1 coloured by chain, top view.
Source organism: Magnetospirillum gryphiswaldense MSR-1 v2
Primary publication:
Mechanistic insights into metal ion activation and operator recognition by the ferric uptake regulator.
Deng Z, Wang Q, Liu Z, Zhang M, Machado AC, Chiu TP, Feng C, Zhang Q, Yu L, Qi L, Zheng J, Wang X, Huo X, Qi X, Li X, Wu W, Rohs R, Li Y, Chen Z
Nat Commun 6 7642 (2015)
PMID: 26134419

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118086 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Ferric uptake regulation protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 145 amino acids
Theoretical weight: 16.63 KDa
Source organism: Magnetospirillum gryphiswaldense MSR-1 v2
Expression system: Escherichia coli
UniProt:
  • Canonical: V6F4Q0 (Residues: 1-143; Coverage: 100%)
Gene names: Fur, MGMSRv2__3137, fur
Sequence domains: Ferric uptake regulator family
Structure domains:
DNA (5'-D(*CP*GP*CP*GP*AP*TP*AP*AP*TP*GP*AP*TP*AP*AP*TP*CP*AP*TP*TP*AP*TP*CP*CP*GP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 25 nucleotides
Theoretical weight: 7.64 KDa
Source organism: Magnetospirillum gryphiswaldense MSR-1 v2
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: C2
Unit cell:
a: 159.502Å b: 42.855Å c: 60.547Å
α: 90° β: 94.91° γ: 90°
R-values:
R R work R free
0.227 0.226 0.249
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

Metal homeostasis and resistance in bacteria.
Chandrangsu et al. (2017)
10 more

11 mentions without citation

Metallochaperones and metalloregulation in bacteria.
Capdevila et al. (2017)
10 more