› 4rb4

X-ray diffraction
3.88Å resolution

Crystal structure of dodecameric iron-containing heptosyltransferase TibC in complex with ADP-D-beta-D-heptose at 3.9 angstrom resolution

Released:
DOI: 10.2210/pdb4rb4/pdb
PDB entry 4rb4 coloured by chain, front view.
PDB entry 4rb4 coloured by chain, side view.
PDB entry 4rb4 coloured by chain, top view.
Source organism: Escherichia coli DEC13E
Primary publication:
A structural mechanism for bacterial autotransporter glycosylation by a dodecameric heptosyltransferase family.
Yao Q, Lu Q, Wan X, Song F, Xu Y, Hu M, Zamyatina A, Liu X, Huang N, Zhu P, Shao F
Elife 3 (2014)
PMID: 25310236

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165150 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyltransferase tibC Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 406 amino acids
Theoretical weight: 46.33 KDa
Source organism: Escherichia coli DEC13E
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand FE 12 x FE
Ligand AQH 12 x AQH
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 88.292Å b: 313.196Å c: 164.695Å
α: 90° β: 101.26° γ: 90°
R-values:
R R work R free
0.283 0.282 0.295
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Glycosyltransferases: mechanisms and applications in natural product development.
Liang et al. (2015)
4 more

2 mentions without citation

A structural mechanism for bacterial autotransporter glycosylation by a dodecameric heptosyltransferase family.
Yao et al. (2014)
1 more