› 4rdb

X-ray diffraction
1.45Å resolution

Crystal structure of an immunoreactive 32 kDa antigen PG49 (PG_0181) from Porphyromonas gingivalis W83 at 1.45 A resolution (PSI Community Target, Nakayama)

Released:
DOI: 10.2210/pdb4rdb/pdb
PDB entry 4rdb coloured by chain, front view.
PDB entry 4rdb coloured by chain, side view.
PDB entry 4rdb coloured by chain, top view.
Source organism: Porphyromonas gingivalis W83
Primary publication:
A Distinct Type of Pilus from the Human Microbiome.
Xu Q, Shoji M, Shibata S, Naito M, Sato K, Elsliger MA, Grant JC, Axelrod HL, Chiu HJ, Farr CL, Jaroszewski L, Knuth MW, Deacon AM, Godzik A, Lesley SA, Curtis MA, Nakayama K, Wilson IA
Cell 165 690-703 (2016)
PMID: 27062925

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181781 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Minor fimbrium tip subunit MfA4 Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 34.13 KDa
Source organism: Porphyromonas gingivalis W83
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7MXK0 (Residues: 30-333; Coverage: 97%)
Gene names: PG_0181, mfA4
Sequence domains: Major fimbrial subunit protein (FimA)
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:
Ligand EDO 13 x EDO
Ligand ACN 1 x ACN
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 39.636Å b: 86.821Å c: 90.645Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.119 0.117 0.157
Expression system: Escherichia coli

Quick links

Citations

10 review citations

The Type IX Secretion System (T9SS): Highlights and Recent Insights into Its Structure and Function.
Lasica et al. (2017)
9 more

1 mention without citation

Structure of the fimbrial protein Mfa4 from Porphyromonas gingivalis in its precursor form: implications for a donor-strand complementation mechanism.
Kloppsteck et al. (2016)