› 4re4

X-ray diffraction
2.29Å resolution

Different transition state conformations for the hydrolysis of beta-mannosides and beta-glucosides in the rice Os7BGlu26 family GH1 beta-mannosidase/beta-glucosidase

Released:
DOI: 10.2210/pdb4re4/pdb
PDB entry 4re4 coloured by chain, front view.
PDB entry 4re4 coloured by chain, side view.
PDB entry 4re4 coloured by chain, top view.
Source organism: Oryza sativa Indica Group
Primary publication:
A Single Glycosidase Harnesses Different Pyranoside Ring Transition State Conformations for Hydrolysis of Mannosides and Glucosides
Tankrathok A, Iglesias-Fernandez J, Williams RJ, Pengthaisong S, Baiya S, Hakki Z, Robinson RC, Hrmova M, Rovira C, Williams SJ, Ketudat Cairns JR
ACS Catalysis 5 6041-6051 (2015)

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106989 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucosidase Chain: A
Molecule details ›
Chain: A
Length: 503 amino acids
Theoretical weight: 57.7 KDa
Source organism: Oryza sativa Indica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: A2YPH1 (Residues: 28-510; Coverage: 100%)
Gene name: OsI_27163
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

3 bound ligands:
Ligand IFM 1 x IFM
Ligand GOL 4 x GOL
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 68.512Å b: 72.076Å c: 135.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.168 0.21
Expression system: Escherichia coli

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