4res

X-ray diffraction
3.41Å resolution

Crystal structure of the Na,K-ATPase E2P-bufalin complex with bound potassium

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-138328 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Sodium/potassium-transporting ATPase subunit alpha-1 Chains: A, C
Sodium/potassium-transporting ATPase subunit beta-1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 303 amino acids
Theoretical weight: 35.2 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P05027 (Residues: 1-303; Coverage: 100%)
Gene name: ATP1B1
Sequence domains: Sodium / potassium ATPase beta chain
Structure domains:
FXYD domain-containing ion transport regulator Chains: E, G
Molecule details ›
Chains: E, G
Length: 65 amino acids
Theoretical weight: 7.09 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q58K79 (Residues: 1-65; Coverage: 100%)
Gene name: FXYD2
Sequence domains: ATP1G1/PLM/MAT8 family

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: GLC, FRU
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 65.91Å b: 240.27Å c: 152.7Å
α: 90° β: 102.28° γ: 90°
R-values:
R R work R free
0.247 0.245 0.288