› 4rfb

X-ray diffraction
1.93Å resolution

1.93 Angstrom Crystal Structure of Superantigen-like Protein from Staphylococcus aureus in Complex with Sialyl-Lewis X.

Released:
DOI: 10.2210/pdb4rfb/pdb
PDB entry 4rfb coloured by chain, front view.
PDB entry 4rfb coloured by chain, side view.
PDB entry 4rfb coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
Entry authors: Minasov G, Nocadello S, Shuvalova L, Filippova EV, Halavaty A, Dubrovska I, Bagnoli F, Falugi F, Bottomley M, Grandi G, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173533 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Staphylococcal superantigen-like OB-fold domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 218 amino acids
Theoretical weight: 25.66 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2G1S5 (Residues: 31-231; Coverage: 100%)
Gene name: SAOUHSC_00391
Sequence domains: Staphylococcal superantigen-like OB-fold domain
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GAL, SIA, FUC
Carbohydrate polymer
4 bound ligands:
Ligand CL 6 x CL
Ligand PEG 3 x PEG
Ligand PGE 1 x PGE
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 42.64Å b: 108.199Å c: 167.655Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.218
Expression system: Escherichia coli

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