› 4rgf

X-ray diffraction
3.2Å resolution

Crystal structure of the in-line aligned env22 twister ribozyme soaked with Mn2+

Released:
DOI: 10.2210/pdb4rgf/pdb
PDB entry 4rgf coloured by chain, front view.
PDB entry 4rgf coloured by chain, side view.
PDB entry 4rgf coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
In-line alignment and Mg²⁺ coordination at the cleavage site of the env22 twister ribozyme.
Ren A, Košutić M, Rajashankar KR, Frener M, Santner T, Westhof E, Micura R, Patel DJ
Nat Commun 5 5534 (2014)
PMID: 25410397

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196870 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
env22 twister ribozyme Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 56 nucleotides
Theoretical weight: 18.01 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand MN 11 x MN
Ligand K 14 x K
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P6122
Unit cell:
a: 64.299Å b: 64.299Å c: 584.991Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.231 0.277
Expression system: Not provided

Quick links

Citations

11 review citations

RNA Structural Dynamics As Captured by Molecular Simulations: A Comprehensive Overview.
Šponer et al. (2018)
10 more

2 mentions without citation

In-line alignment and Mg²⁺ coordination at the cleavage site of the env22 twister ribozyme.
Ren et al. (2014)
1 more