› 4rgy

X-ray diffraction
1.4Å resolution

Structural and functional analysis of a low-temperature-active alkaline esterase from South China Sea marine sediment microbial metagenomic library

Released:
DOI: 10.2210/pdb4rgy/pdb
PDB entry 4rgy coloured by chain, front view.
PDB entry 4rgy coloured by chain, side view.
PDB entry 4rgy coloured by chain, top view.
Source organism: uncultured bacterium FLS12
Primary publication:
Structural and functional analysis of a low-temperature-active alkaline esterase from South China Sea marine sediment microbial metagenomic library.
Hu Y, Liu Y, Li J, Feng Y, Lu N, Zhu B, Xue S
J Ind Microbiol Biotechnol 42 1449-61 (2015)
PMID: 26350078

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110779 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Esterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 271 amino acids
Theoretical weight: 30.27 KDa
Source organism: uncultured bacterium FLS12
Expression system: Escherichia coli
UniProt:
  • Canonical: B8Y562 (Residues: 1-270; Coverage: 100%)
Sequence domains: Putative esterase
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 133.459Å b: 63.767Å c: 88.688Å
α: 90° β: 127.11° γ: 90°
R-values:
R R work R free
0.181 0.176 0.181
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Metagenomics: novel enzymes from non-culturable microbes.
Berini et al. (2017)
1 more

3 mentions without citation

Structural insight into a CE15 esterase from the marine bacterial metagenome.
De Santi et al. (2017)
2 more