› 4rhb

X-ray diffraction
3.36Å resolution

Crystal structure of the lipopolysaccharide assembly complex LptD-LptE from the Escherichia coli outer membrane

Released:
DOI: 10.2210/pdb4rhb/pdb
PDB entry 4rhb coloured by chain, front view.
PDB entry 4rhb coloured by chain, side view.
PDB entry 4rhb coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Malojcic G, Garner RA, Kahne D

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142341 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
LPS-assembly protein LptD Chains: A, C
Molecule details ›
Chains: A, C
Length: 614 amino acids
Theoretical weight: 71.23 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P31554 (Residues: 203-784; Coverage: 77%)
Gene names: JW0053, b0054, imp, lptD, ostA, yabG
Sequence domains: LPS transport system D
LPS-assembly lipoprotein LptE Chains: B, D
Molecule details ›
Chains: B, D
Length: 175 amino acids
Theoretical weight: 19.48 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ADC1 (Residues: 19-193; Coverage: 100%)
Gene names: JW0636, b0641, lptE, rlpB
Sequence domains: Lipopolysaccharide-assembly
Structure domains: Lipoprotein like domain

Ligands and Environments

2 bound ligands:
Ligand CL 4 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21212
Unit cell:
a: 128.04Å b: 136.62Å c: 108.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.261 0.257 0.338
Expression system: Escherichia coli

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