› 4rhk

X-ray diffraction
2.38Å resolution

Crystal structure of T. brucei arginase-like protein in an oxidized form

Released:
DOI: 10.2210/pdb4rhk/pdb
PDB entry 4rhk coloured by chain, front view.
PDB entry 4rhk coloured by chain, side view.
PDB entry 4rhk coloured by chain, top view.
Source organism: Trypanosoma brucei brucei TREU927
Primary publication:
Crystal structure of an arginase-like protein from Trypanosoma brucei that evolved without a binuclear manganese cluster.
Hai Y, Kerkhoven EJ, Barrett MP, Christianson DW
Biochemistry 54 458-71 (2015)
PMID: 25536859

Function and Biology Details

Reaction catalysed: 
L-arginine + H(2)O = L-ornithine + urea
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginase, putative Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 38.73 KDa
Source organism: Trypanosoma brucei brucei TREU927
Expression system: Escherichia coli
UniProt:
  • Canonical: Q581Y0 (Residues: 1-331; Coverage: 100%)
Gene names: Tb08.26N11.490, Tb927.8.2020
Sequence domains: Arginase family
Structure domains: Ureohydrolase domain

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: R32
Unit cell:
a: 153.148Å b: 153.148Å c: 85.426Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.227 0.256
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Comparative Metabolism of Free-living Bodo saltans and Parasitic Trypanosomatids.
Opperdoes et al. (2016)
4 more

1 mention without citation

Crystal structure of an arginase-like protein from Trypanosoma brucei that evolved without a binuclear manganese cluster.
Hai et al. (2015)