› 4rhs

X-ray diffraction
1.92Å resolution

Crystal structure of GD2 bound PltB

Released:
DOI: 10.2210/pdb4rhs/pdb
PDB entry 4rhs coloured by chain, front view.
PDB entry 4rhs coloured by chain, side view.
PDB entry 4rhs coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhi
Primary publication:
Host adaptation of a bacterial toxin from the human pathogen Salmonella Typhi.
Deng L, Song J, Gao X, Wang J, Yu H, Chen X, Varki N, Naito-Matsui Y, Galán JE, Varki A
Cell 159 1290-9 (2014)
PMID: 25480294

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187168 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Bordetella pertussis toxin B subunit 2/3 C-terminal domain-containing protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 122 amino acids
Theoretical weight: 13.63 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8Z6A3 (Residues: 24-137; Coverage: 100%)
Gene names: CAJ76_21360, CAJ80_20450, CAJ85_21845, CAL67_19310, CB224_21995, CC884_21770, CC907_20725, CC972_22120, D4Z37_22870, D6Q30_20400, DKA88_22915, DNM71_22615, DNV09_22455, DS261_23220, DUQ91_23125, DUV11_22855, DVG36_22585, EBC38_22935, EID90_22310, ELQ62_22905, EU445_23060, EVI00_22265, F9R11_22575, F9Y21_22590, FI137_21150, G2227_22525, G2244_22955, G3982_004274, G4I71_004183, G4L28_004281, G4P30_004279, G4Y41_004487, G4Y55_004474, G9264_003909, G9C68_004513, GDI29_22175, GND71_004363, K3U68_05550, STY1891, ZZ17_21790, pltB, t1107
Sequence domains: Pertussis toxin, subunit 2 and 3, C-terminal domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL, SIA
Carbohydrate polymer
1 bound ligand:
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 68.257Å b: 96.751Å c: 104.002Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.205
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Biological roles of glycans.
Varki A. (2017)
24 more

3 mentions without citation

Host adaptation of a bacterial toxin from the human pathogen Salmonella Typhi.
Deng et al. (2014)
2 more