› 4ri5

X-ray diffraction
1.26Å resolution

Crystal structure of PTPN3 (PTPH1) D811E mutant in complex with metavanadate

Released:
DOI: 10.2210/pdb4ri5/pdb
PDB entry 4ri5 coloured by chain, front view.
PDB entry 4ri5 coloured by chain, side view.
PDB entry 4ri5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Substrate specificity and plasticity of FERM-containing protein tyrosine phosphatases.
Chen KE, Li MY, Chou CC, Ho MR, Chen GC, Meng TC, Wang AH
Structure 23 653-64 (2015)
PMID: 25728925

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 306 amino acids
Theoretical weight: 34.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P26045 (Residues: 628-909; Coverage: 31%)
Gene names: PTPH1, PTPN3
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand VN4 2 x VN4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: C2221
Unit cell:
a: 95.162Å b: 96.071Å c: 141.722Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.159 0.173
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The progress of research into pseudophosphatases.
Liu et al. (2022)
9 other articles

1 mention without citation

Interaction of Vanadium Complexes with Proteins: Revisiting the Reported Structures in the Protein Data Bank (PDB) since 2015.
Santos et al. (2023)