› 4ri9

X-ray diffraction
2.9Å resolution

FAN1 Nuclease bound to 5' phosphorylated p(dT)/3'(dT-dT-dT-dT-dT-dT-dT-dT) double flap DNA

Released:
DOI: 10.2210/pdb4ri9/pdb
PDB entry 4ri9 coloured by chain, front view.
PDB entry 4ri9 coloured by chain, side view.
PDB entry 4ri9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
DNA repair. Mechanism of DNA interstrand cross-link processing by repair nuclease FAN1.
Wang R, Persky NS, Yoo B, Ouerfelli O, Smogorzewska A, Elledge SJ, Pavletich NP
Science 346 1127-30 (2014)
PMID: 25430771

Function and Biology Details

Reaction catalysed: 
Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118099 (preferred)
Entry contents:
1 distinct polypeptide molecule
4 distinct DNA molecules
Macromolecules (5 distinct):
Fanconi-associated nuclease 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 652 amino acids
Theoretical weight: 74.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y2M0 (Residues: 370-1017; Coverage: 63%)
Gene names: FAN1, KIAA1018, MTMR15
Sequence domains:
DNA (5'-D(P*TP*AP*GP*CP*CP*AP*CP*GP*CP*CP*T)-3') Chains: S, W
Molecule details ›
Chains: S, W
Length: 11 nucleotides
Theoretical weight: 3.29 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*AP*GP*AP*CP*TP*CP*CP*TP*CP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chains: U, Z
Molecule details ›
Chains: U, Z
Length: 17 nucleotides
Theoretical weight: 5.11 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*GP*CP*TP*GP*AP*GP*GP*AP*GP*TP*CP*T)-3') Chains: V, X
Molecule details ›
Chains: V, X
Length: 12 nucleotides
Theoretical weight: 3.72 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*TP*TP*TP*TP*TP*TP*GP*AP*GP*GP*CP*GP*TP*G)-3') Chains: T, Y
Molecule details ›
Chains: T, Y
Length: 14 nucleotides
Theoretical weight: 4.33 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand BA 2 x BA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 66.865Å b: 211.574Å c: 69.273Å
α: 90° β: 99.15° γ: 90°
R-values:
R R work R free
0.228 0.227 0.266
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

14 review citations

Fanconi anaemia and cancer: an intricate relationship.
Nalepa et al. (2018)
13 more

1 mention without citation

DNA repair. Mechanism of DNA interstrand cross-link processing by repair nuclease FAN1.
Wang et al. (2014)