› 4rk9

X-ray diffraction
2.15Å resolution

CRYSTAL STRUCTURE OF SUGAR TRANSPORTER BL01359 FROM Bacillus licheniformis, TARGET EFI-510856, IN COMPLEX WITH STACHYOSE

Released:
DOI: 10.2210/pdb4rk9/pdb
PDB entry 4rk9 coloured by chain, front view.
PDB entry 4rk9 coloured by chain, side view.
PDB entry 4rk9 coloured by chain, top view.
Source organism: Bacillus licheniformis DSM 13 = ATCC 14580
Entry authors: Patskovsky Y, Toro R, Bhosle R, Al Obaidi N, Chamala S, Attonito JD, Scott Glenn A, Chowdhury S, Lafleur J, Siedel RD, Hillerich B, Love J, Whalen KL, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179218 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Multiple sugar-binding protein MsmE Chains: A, B
Molecule details ›
Chains: A, B
Length: 401 amino acids
Theoretical weight: 45.5 KDa
Source organism: Bacillus licheniformis DSM 13 = ATCC 14580
Expression system: Escherichia coli
UniProt:
  • Canonical: Q65LL6 (Residues: 19-417; Coverage: 100%)
Gene names: BL01359, msmE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, GLA, FRU
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 74.332Å b: 85.916Å c: 132.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.272
Expression system: Escherichia coli

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