› 4rni

X-ray diffraction
2.17Å resolution

PaMorA dimeric phosphodiesterase. apo form

Released:
DOI: 10.2210/pdb4rni/pdb
PDB entry 4rni coloured by chain, front view.
PDB entry 4rni coloured by chain, side view.
PDB entry 4rni coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Formation and dimerization of the phosphodiesterase active site of the Pseudomonas aeruginosa MorA, a bi-functional c-di-GMP regulator.
Phippen CW, Mikolajek H, Schlaefli HG, Keevil CW, Webb JS, Tews I
FEBS Lett 588 4631-6 (2014)
PMID: 25447517

Function and Biology Details

Reaction catalysed: 
Cyclic di-3',5'-guanylate + H(2)O = 5'-phosphoguanylyl(3'->5')guanosine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191014 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Motility regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 31.73 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HVI8 (Residues: 1145-1409; Coverage: 19%)
Gene names: PA4601, morA
Sequence domains: EAL domain
Structure domains: EAL domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Unit cell:
a: 35.754Å b: 94.466Å c: 154.703Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.181 0.238
Expression system: Escherichia coli

Quick links

Citations

2 review citations

C-di-GMP Synthesis: Structural Aspects of Evolution, Catalysis and Regulation.
Schirmer T. (2016)
1 more

2 mentions without citation

Dimerisation induced formation of the active site and the identification of three metal sites in EAL-phosphodiesterases.
Bellini et al. (2017)
1 more