› 4rnp

X-ray diffraction
3Å resolution

BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY

Released:
DOI: 10.2210/pdb4rnp/pdb
PDB entry 4rnp coloured by chain, front view.
PDB entry 4rnp coloured by chain, side view.
PDB entry 4rnp coloured by chain, top view.
Source organism: Escherichia phage T7
Primary publication:
Crystal structure of bacteriophage T7 RNA polymerase at 3.3 A resolution.
Sousa R, Chung YJ, Rose JP, Wang BC
Nature 364 593-9 (1993)
PMID: 7688864

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132725 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
T7 RNA polymerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 883 amino acids
Theoretical weight: 98.98 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P00573 (Residues: 1-883; Coverage: 100%)
Gene name: 1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 112.267Å b: 137.681Å c: 122.566Å
α: 90° β: 96.36° γ: 90°
Expression system: Escherichia coli

Quick links

Citations

34 review citations

Comparison of the replication of positive-stranded RNA viruses of plants and animals.
Buck KW. (1996)
33 more

7 mentions without citation

Computational simulation strategies for analysis of multisubunit RNA polymerases.
Wang et al. (2013)
6 more