› 4ros

X-ray diffraction
1.95Å resolution

Crystal structure of Methylobacterium extorquens malate dehydrogenase complexed with oxaloacetate and adenosine-5-diphosphoribose

Released:
DOI: 10.2210/pdb4ros/pdb
PDB entry 4ros coloured by chain, front view.
PDB entry 4ros coloured by chain, side view.
PDB entry 4ros coloured by chain, top view.
Source organism: Methylorubrum extorquens PA1
Entry authors: Gonzalez JM, Marti-Arbona R, Unkefer CJ

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = oxaloacetate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109053 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 33.68 KDa
Source organism: Methylorubrum extorquens PA1
Expression system: Escherichia coli
UniProt:
  • Canonical: A9W386 (Residues: 1-320; Coverage: 100%)
Gene names: Mext_1643, mdh
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand OAA 1 x OAA
Ligand APR 1 x APR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P6422
Unit cell:
a: 108.396Å b: 108.396Å c: 104.014Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.168 0.199
Expression system: Escherichia coli

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