› 4rp5

X-ray diffraction
1.65Å resolution

Crystal Structure of the L27 domain of Discs Large 1 (target ID NYSGRC-010766) from Drosophila melanogaster (space group P21)

Released:
DOI: 10.2210/pdb4rp5/pdb
PDB entry 4rp5 coloured by chain, front view.
PDB entry 4rp5 coloured by chain, side view.
PDB entry 4rp5 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structures of the L27 Domain of Disc Large Homologue 1 Protein Illustrate a Self-Assembly Module.
Ghosh A, Ramagopal UA, Bonanno JB, Brenowitz M, Almo SC
Biochemistry 57 1293-1305 (2018)
PMID: 29261291

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151872 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Disks large 1 tumor suppressor protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 98 amino acids
Theoretical weight: 11.48 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31007 (Residues: 1-97; Coverage: 10%)
Gene names: CG1725, dlg1, l(1)dlg1
Sequence domains: L27_1

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 28.546Å b: 47.818Å c: 64.424Å
α: 90° β: 95.95° γ: 90°
R-values:
R R work R free
0.198 0.182 0.213
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Hippo Signaling in Cancer: Lessons From Drosophila Models.
Snigdha et al. (2019)
3 more

1 mention without citation

Structures of the L27 Domain of Disc Large Homologue 1 Protein Illustrate a Self-Assembly Module.
Ghosh et al. (2018)