› 4rp9

X-ray diffraction
1.65Å resolution

Bacterial vitamin C transporter UlaA/SgaT in C2 form

Released:
DOI: 10.2210/pdb4rp9/pdb
PDB entry 4rp9 coloured by chain, front view.
PDB entry 4rp9 coloured by chain, side view.
PDB entry 4rp9 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of a phosphorylation-coupled vitamin C transporter.
Luo P, Yu X, Wang W, Fan S, Li X, Wang J
Nat Struct Mol Biol 22 238-41 (2015)
PMID: 25686089

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153860 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ascorbate-specific PTS system EIIC component Chain: A
Molecule details ›
Chain: A
Length: 465 amino acids
Theoretical weight: 50.77 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P39301 (Residues: 1-465; Coverage: 100%)
Gene names: JW5744, b4193, sgaT, ulaA, yjfS
Sequence domains: PTS system sugar-specific permease component

Ligands and Environments

4 bound ligands:
Ligand ASC 1 x ASC
Ligand BNG 3 x BNG
Ligand TGL 1 x TGL
Ligand 1PE 3 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 113.663Å b: 85.812Å c: 83.236Å
α: 90° β: 127.97° γ: 90°
R-values:
R R work R free
0.136 0.134 0.174
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
2 more

8 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
7 more