› 4rph

X-ray diffraction
2.6Å resolution

Crystal structure of Micobacterium tuberculosis UDP-Galactopyranose mutase in complex with substrate UDP-Galp (reduced)

Released:
DOI: 10.2210/pdb4rph/pdb
PDB entry 4rph coloured by chain, front view.
PDB entry 4rph coloured by chain, side view.
PDB entry 4rph coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structural basis of ligand binding to UDP-galactopyranose mutase from Mycobacterium tuberculosis using substrate and tetrafluorinated substrate analogues.
van Straaten KE, Kuttiyatveetil JR, Sevrain CM, Villaume SA, Jiménez-Barbero J, Linclau B, Vincent SP, Sanders DA
J Am Chem Soc 137 1230-44 (2015)
PMID: 25562380

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-galactopyranose mutase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 399 amino acids
Theoretical weight: 45.93 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WIQ1 (Residues: 1-399; Coverage: 100%)
Gene names: Rv3809c, glf
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand FAD 3 x FAD
1 bound ligand:
Ligand GDU 3 x GDU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2
Unit cell:
a: 171.73Å b: 99.47Å c: 100.24Å
α: 90° β: 110.17° γ: 90°
R-values:
R R work R free
0.21 0.208 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Biosynthesis of Galactan in Mycobacterium tuberculosis as a Viable TB Drug Target?
Konyariková et al. (2020)
1 more

1 mention without citation

The Search for Herbal Antibiotics: An In-Silico Investigation of Antibacterial Phytochemicals.
Snow Setzer et al. (2016)