› 4rpm

X-ray diffraction
1.4Å resolution

Crystal structure of the SAT domain from the non-reducing fungal polyketide synthase CazM with bound hexanoyl

Released:
DOI: 10.2210/pdb4rpm/pdb
PDB entry 4rpm coloured by chain, front view.
PDB entry 4rpm coloured by chain, side view.
PDB entry 4rpm coloured by chain, top view.
Source organism: Chaetomium globosum CBS 148.51
Primary publication:
Biochemical and Structural Basis for Controlling Chemical Modularity in Fungal Polyketide Biosynthesis.
Winter JM, Cascio D, Dietrich D, Sato M, Watanabe K, Sawaya MR, Vederas JC, Tang Y
J Am Chem Soc 137 9885-93 (2015)
PMID: 26172141

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173617 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ketosynthase family 3 (KS3) domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 406 amino acids
Theoretical weight: 44.58 KDa
Source organism: Chaetomium globosum CBS 148.51
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2GWK9 (Residues: 2-113, 114-125, 126-378; Coverage: 32%)
Gene name: CHGG_07645
Sequence domains: Starter unit:ACP transacylase in aflatoxin biosynthesis

Ligands and Environments

2 bound ligands:
Ligand 6NA 1 x 6NA
Ligand HXC 1 x HXC
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 45.67Å b: 52.36Å c: 168.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

The architectures of iterative type I PKS and FAS.
Herbst et al. (2018)
9 more

1 mention without citation

Biochemical and Structural Basis for Controlling Chemical Modularity in Fungal Polyketide Biosynthesis.
Winter et al. (2015)