› 4rsq

X-ray diffraction
2.9Å resolution

2.9A resolution structure of SRPN2 (K198C/E359C) from Anopheles gambiae

Released:
DOI: 10.2210/pdb4rsq/pdb
Model geometry
Fit model/data
PDB entry 4rsq coloured by chain, front view.
PDB entry 4rsq coloured by chain, side view.
PDB entry 4rsq coloured by chain, top view.
Source organism: Anopheles gambiae
Primary publication:
Structural and inhibitory effects of hinge loop mutagenesis in serpin-2 from the malaria vector Anopheles gambiae.
Zhang X, Meekins DA, An C, Zolkiewski M, Battaile KP, Kanost MR, Lovell S, Michel K
J Biol Chem 290 2946-56 (2015)
PMID: 25525260

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181881 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease inhibitor 2 Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 397 amino acids
Theoretical weight: 45.31 KDa
Source organism: Anopheles gambiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7QIJ8 (Residues: 22-409; Coverage: 100%)
Gene names: 1270169, AgaP_AGAP006911, SRPN2
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 97.925Å b: 164.392Å c: 186.181Å
α: 90° β: 90.02° γ: 90°
R-values:
R R work R free
0.197 0.194 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Serpins in arthropod biology.
Meekins et al. (2017)
5 other articles

1 mention without citation

Structural and inhibitory effects of hinge loop mutagenesis in serpin-2 from the malaria vector Anopheles gambiae.
Zhang et al. (2015)