› 4rwo

X-ray diffraction
2.2Å resolution

Crystal structure of the porcine OAS1 L149R mutant in complex with dsRNA and ApCpp in the AMP donor position

Released:
DOI: 10.2210/pdb4rwo/pdb
PDB entry 4rwo coloured by chain, front view.
PDB entry 4rwo coloured by chain, side view.
PDB entry 4rwo coloured by chain, top view.
Source organisms:
Primary publication:
The Activation Mechanism of 2'-5'-Oligoadenylate Synthetase Gives New Insights Into OAS/cGAS Triggers of Innate Immunity.
Lohöfener J, Steinke N, Kay-Fedorov P, Baruch P, Nikulin A, Tishchenko S, Manstein DJ, Fedorov R
Structure 23 851-862 (2015)
PMID: 25892109

Function and Biology Details

Reaction catalysed: 
3 ATP = pppA2'p5'A2'p5'A + 2 diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173358 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
2'-5'-oligoadenylate synthase 1 Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 41.32 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q29599 (Residues: 1-349; Coverage: 100%)
Gene name: OAS1
Sequence domains:
Structure domains:
RNA (5'-R(*GP*GP*CP*UP*UP*UP*UP*GP*AP*CP*CP*UP*UP*UP*AP*UP*GP*AP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 19 nucleotides
Theoretical weight: 6.02 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (5'-R(*UP*UP*CP*AP*UP*AP*AP*AP*GP*GP*UP*CP*AP*AP*AP*AP*GP*CP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 6.07 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand APC 1 x APC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P43212
Unit cell:
a: 73.1Å b: 73.1Å c: 208.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.237
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

Dance with the Devil: Stress Granules and Signaling in Antiviral Responses.
Eiermann et al. (2020)
7 more

3 mentions without citation

Bacterial cGAS-like enzymes synthesize diverse nucleotide signals.
Whiteley et al. (2019)
2 more