› 4s05

X-ray diffraction
3.8Å resolution

Crystal structure of Klebsiella pneumoniae PmrA in complex with PmrA box DNA

Released:
DOI: 10.2210/pdb4s05/pdb
PDB entry 4s05 coloured by chain, front view.
PDB entry 4s05 coloured by chain, side view.
PDB entry 4s05 coloured by chain, top view.
Source organisms:
Primary publication:
Structure and dynamics of polymyxin-resistance-associated response regulator PmrA in complex with promoter DNA.
Lou YC, Weng TH, Li YC, Kao YF, Lin WF, Peng HL, Chou SH, Hsiao CD, Chen C
Nat Commun 6 8838 (2015)
PMID: 26564787

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102625 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding transcriptional regulator BasR Chains: A, B
Molecule details ›
Chains: A, B
Length: 232 amino acids
Theoretical weight: 26.31 KDa
Source organism: Klebsiella pneumoniae JM45
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0R4I965 (Residues: 1-223; Coverage: 96%)
Gene name: N559_3529
Sequence domains:
Structure domains:
DNA (26-MER) Chain: C
Molecule details ›
Chain: C
Length: 26 nucleotides
Theoretical weight: 7.96 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (26-MER) Chain: D
Molecule details ›
Chain: D
Length: 26 nucleotides
Theoretical weight: 8.01 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand BEF 2 x BEF
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P3121
Unit cell:
a: 162.64Å b: 162.64Å c: 131.707Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.178 0.228
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Regulating polymyxin resistance in Gram-negative bacteria: roles of two-component systems PhoPQ and PmrAB.
Huang et al. (2020)
2 more

10 mentions without citation

Structure and dynamics of polymyxin-resistance-associated response regulator PmrA in complex with promoter DNA.
Lou et al. (2015)
9 more