› 4s0f

X-ray diffraction
5.52Å resolution

Crystal structure of the peptidase-containing ABC transporter PCAT1 E648Q mutant complexed with ATPgS in an occluded conformation

Released:
DOI: 10.2210/pdb4s0f/pdb
PDB entry 4s0f coloured by chain, front view.
PDB entry 4s0f coloured by chain, side view.
PDB entry 4s0f coloured by chain, top view.
Source organism: Acetivibrio thermocellus ATCC 27405
Primary publication:
Crystal structures of a polypeptide processing and secretion transporter.
Lin DY, Huang S, Chen J
Nature 523 425-30 (2015)
PMID: 26201595

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107034 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABC-type bacteriocin transporter Chains: A, B
Molecule details ›
Chains: A, B
Length: 730 amino acids
Theoretical weight: 81.18 KDa
Source organism: Acetivibrio thermocellus ATCC 27405
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3DCU1 (Residues: 1-727; Coverage: 100%)
Gene name: Cthe_0534
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand AGS 2 x AGS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P42212
Unit cell:
a: 230Å b: 230Å c: 89.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.301 0.3 0.314
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Mechanistic diversity in ATP-binding cassette (ABC) transporters.
Locher KP. (2016)
11 more

10 mentions without citation

Mechanistic Understanding of Lanthipeptide Biosynthetic Enzymes.
Repka et al. (2017)
9 more