› 4s1w

X-ray diffraction
1.65Å resolution

Structure of a putative Glutamine--Fructose-6-Phosphate Aminotransferase from Staphylococcus aureus subsp. aureus Mu50

Released:
DOI: 10.2210/pdb4s1w/pdb
Model geometry
Fit model/data
PDB entry 4s1w coloured by chain, front view.
PDB entry 4s1w coloured by chain, side view.
PDB entry 4s1w coloured by chain, top view.
Source organism: Staphylococcus aureus
Entry authors: Filippova EV, Shuvalova L, Kiryukhina O, Jedrzejczak R, Babnigg G, Rubin E, Sacchettini J, Joachimiak A, Anderson WF, Midwest Center for Structural Genomics (MCSG), Structures of Mtb Proteins Conferring Susceptibility to Known Mtb Inhibitors (MTBI)

Function and Biology Details

Reaction catalysed: 
L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102252 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] Chains: A, B
Molecule details ›
Chains: A, B
Length: 358 amino acids
Theoretical weight: 39.43 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0J9X200 (Residues: 1-358; Coverage: 100%)
Sequence domains: SIS domain
Structure domains: Glucose-6-phosphate isomerase like protein; domain 1

Ligands and Environments

1 bound ligand:
Ligand PEG 2 x PEG
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 115.245Å b: 61.617Å c: 104.69Å
α: 90° β: 112.79° γ: 90°
R-values:
R R work R free
0.161 0.16 0.192
Expression system: Escherichia coli BL21(DE3)

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