› 4s21

X-ray diffraction
3.25Å resolution

Crystal structure of the photosensory core module of bacteriophytochrome RPA3015 from R. palustris

Released:
DOI: 10.2210/pdb4s21/pdb
PDB entry 4s21 coloured by chain, front view.
PDB entry 4s21 coloured by chain, side view.
PDB entry 4s21 coloured by chain, top view.
Source organism: Rhodopseudomonas palustris CGA009
Primary publication:
Light Signaling Mechanism of Two Tandem Bacteriophytochromes.
Yang X, Stojković EA, Ozarowski WB, Kuk J, Davydova E, Moffat K
Structure 23 1179-89 (2015)
PMID: 26095026

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179964 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 525 amino acids
Theoretical weight: 57.36 KDa
Source organism: Rhodopseudomonas palustris CGA009
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q6N5G3 (Residues: 1-505; Coverage: 67%)
Gene names: RPA3015, TX73_015615, phyB1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BLA 2 x BLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P61
Unit cell:
a: 175.36Å b: 175.36Å c: 96.19Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.264 0.262 0.316
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Signal transduction in photoreceptor histidine kinases.
Möglich A. (2019)
2 more

3 mentions without citation

Long-range allosteric signaling in red light-regulated diguanylyl cyclases.
Gourinchas et al. (2017)
2 more