› 4s3r

X-ray diffraction
2.1Å resolution

Amylomaltase MalQ from Escherichia coli in complex with the pseudo-heptasaccharide acarviosine-glucose-acarbose

Released:
DOI: 10.2210/pdb4s3r/pdb
PDB entry 4s3r coloured by chain, front view.
PDB entry 4s3r coloured by chain, side view.
PDB entry 4s3r coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural Basis for the Interconversion of Maltodextrins by MalQ, the Amylomaltase of Escherichia coli.
Weiss SC, Skerra A, Schiefner A
J Biol Chem 290 21352-64 (2015)
PMID: 26139606

Function and Biology Details

Reaction catalysed: 
Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147572 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-alpha-glucanotransferase Chain: A
Molecule details ›
Chain: A
Length: 696 amino acids
Theoretical weight: 80.01 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P15977 (Residues: 1-688; Coverage: 99%)
Gene names: JW3379, b3416, malA, malQ
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand 7SA 1 x 7SA
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 65Å b: 75.83Å c: 163.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.205
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

Remarkable evolutionary relatedness among the enzymes and proteins from the α-amylase family.
Janeček et al. (2016)
6 more

5 mentions without citation

Structural Basis for the Interconversion of Maltodextrins by MalQ, the Amylomaltase of Escherichia coli.
Weiss et al. (2015)
4 more