› 4tkr

X-ray diffraction
3Å resolution

Native-SAD phasing for ThiT from Listeria monocytogenes serovar.

Released:
DOI: 10.2210/pdb4tkr/pdb
PDB entry 4tkr coloured by chain, front view.
PDB entry 4tkr coloured by chain, side view.
PDB entry 4tkr coloured by chain, top view.
Source organism: Listeria monocytogenes
Primary publication:
Multi-crystal native SAD analysis at 6 keV.
Liu Q, Guo Y, Chang Y, Cai Z, Assur Z, Mancia F, Greene MI, Hendrickson WA
Acta Crystallogr D Biol Crystallogr 70 2544-57 (2014)
PMID: 25286840

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101030 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Energy-coupled thiamine transporter ThiT Chains: A, B
Molecule details ›
Chains: A, B
Length: 214 amino acids
Theoretical weight: 24.08 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0B8R2U7 (Residues: 1-186; Coverage: 100%)
Gene names: A8L61_06350, AB917_03950, ABZ57_04425, ART25_07095, ARY78_04255, B1N52_07435, B5K54_05330, BCZ19_05280, BCZ21_09580, CAV64_04340, CW845_06970, CW895_10830, D4920_01725, D4B11_00615, D5N24_03695, D7104_07575, DCT16_07795, DQ70_06695, DU018_04470, E1W56_04955, E5F58_09570, EX365_07035, EXZ73_00100, F3300_02945, F6436_12925, F6515_04720, FA835_07720, FLQ76_04315, FLQ91_07680, FLQ97_07070, FNX40_09375, FV747_03130, G3O21_000984, GHH22_03320, GJW51_06670, GQG13_08175, GYR60_09795, GYS09_07700, GYX23_06110, GYY14_05625, KV70_03930, QD52_08055, thiT
Sequence domains: Thiamine transporter protein (Thia_YuaJ)
Structure domains: Arp2/3 complex 21 kDa subunit ARPC3

Ligands and Environments

2 bound ligands:
Ligand TPP 2 x TPP
Ligand BGL 15 x BGL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P3121
Unit cell:
a: 95.647Å b: 95.647Å c: 125.027Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.198 0.234
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Crystallographic phasing from weak anomalous signals.
Liu et al. (2015)
1 more

2 mentions without citation

Be Cautious with Crystal Structures of Membrane Proteins or Complexes Prepared in Detergents.
Guo Y. (2020)
1 more