› 4tl3

X-ray diffraction
2.3Å resolution

Mechanistic insights from the crystal structure of an inward proton-transporting Anabaena sensory rhodopsin mutant

Released:
DOI: 10.2210/pdb4tl3/pdb
PDB entry 4tl3 coloured by chain, front view.
PDB entry 4tl3 coloured by chain, side view.
PDB entry 4tl3 coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Entry authors: Dong BH, Luecke H

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187106 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 226 amino acids
Theoretical weight: 26.06 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8YSC4 (Residues: 1-226; Coverage: 87%)
Gene name: alr3165
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

2 bound ligands:
Ligand RET 2 x RET
Ligand PEE 35 x PEE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 55.265Å b: 104.301Å c: 111.386Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.22 0.25
Expression system: Escherichia coli

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