› 4tlv

X-ray diffraction
1.9Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159975 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosylating toxin CARDS Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 591 amino acids
Theoretical weight: 68.07 KDa
Source organism: Mycoplasma pneumoniae M129
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P75409 (Residues: 1-591; Coverage: 100%)
Gene names: MP464, MPN_372, cards
Sequence domains: Pertussis toxin, subunit 1

Ligands and Environments

3 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 27 x GOL
Ligand ACT 4 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 191.698Å b: 107.407Å c: 222.261Å
α: 90° β: 90.64° γ: 90°
R-values:
R R work R free
0.188 0.186 0.215
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Mycoplasma pneumoniae from the Respiratory Tract and Beyond.
Waites et al. (2017)
11 more

6 mentions without citation

Structure of CARDS toxin, a unique ADP-ribosylating and vacuolating cytotoxin from Mycoplasma pneumoniae.
Becker et al. (2015)
5 more