› 4tmk

X-ray diffraction
1.98Å resolution

COMPLEX OF E. COLI THYMIDYLATE KINASE WITH THE BISUBSTRATE INHIBITOR TP5A

Released:
DOI: 10.2210/pdb4tmk/pdb
PDB entry 4tmk coloured by chain, front view.
PDB entry 4tmk coloured by chain, side view.
PDB entry 4tmk coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for efficient phosphorylation of 3'-azidothymidine monophosphate by Escherichia coli thymidylate kinase.
Lavie A, Ostermann N, Brundiers R, Goody RS, Reinstein J, Konrad M, Schlichting I
Proc Natl Acad Sci U S A 95 14045-50 (1998)
PMID: 9826650

Function and Biology Details

Reaction catalysed: 
ATP + dTMP = ADP + dTDP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-141510 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidylate kinase Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 23.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A720 (Residues: 1-213; Coverage: 100%)
Gene names: JW1084, b1098, tmk, ycfG
Sequence domains: Thymidylate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand T5A 1 x T5A
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: R32
Unit cell:
a: 151.7Å b: 151.7Å c: 74.48Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.204 0.253
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Phosphorylation of nucleosides and nucleoside analogs by mammalian nucleoside monophosphate kinases.
Van Rompay et al. (2000)
41 other articles

10 mentions without citation

Structural basis for efficient phosphorylation of 3'-azidothymidine monophosphate by Escherichia coli thymidylate kinase.
Lavie et al. (1998)
9 more