› 4tn0

X-ray diffraction
2.4Å resolution

Crystal Structure of the C-terminal Periplasmic Domain of Phosphoethanolamine Transferase EptC from Campylobacter jejuni

Released:
DOI: 10.2210/pdb4tn0/pdb
PDB entry 4tn0 coloured by chain, front view.
PDB entry 4tn0 coloured by chain, side view.
PDB entry 4tn0 coloured by chain, top view.
Source organism: Campylobacter jejuni subsp. jejuni HB93-13
Primary publication:
Crystallographic study of the phosphoethanolamine transferase EptC required for polymyxin resistance and motility in Campylobacter jejuni.
Fage CD, Brown DB, Boll JM, Keatinge-Clay AT, Trent MS
Acta Crystallogr D Biol Crystallogr 70 2730-9 (2014)
PMID: 25286856

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165188 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UPF0141 protein yjdB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 325 amino acids
Theoretical weight: 36.88 KDa
Source organism: Campylobacter jejuni subsp. jejuni HB93-13
Expression system: Escherichia coli BL21(DE3)
Structure domains: Alkaline Phosphatase, subunit A

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
1 modified residue:
Ligand TPO 3 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: C2221
Unit cell:
a: 121.85Å b: 183.14Å c: 121.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Resistance to polymyxins in Gram-negative organisms.
Jeannot et al. (2017)
5 more

8 mentions without citation

Phosphoethanolamine Transferases as Drug Discovery Targets for Therapeutic Treatment of Multi-Drug Resistant Pathogenic Gram-Negative Bacteria.
Thai et al. (2023)
7 more