› 4tn3

X-ray diffraction
3.2Å resolution

Structure of the BBox-Coiled-coil region of Rhesus Trim5alpha

Released:
DOI: 10.2210/pdb4tn3/pdb
PDB entry 4tn3 coloured by chain, front view.
PDB entry 4tn3 coloured by chain, side view.
PDB entry 4tn3 coloured by chain, top view.
Source organisms:
Primary publication:
Structural studies of postentry restriction factors reveal antiparallel dimers that enable avid binding to the HIV-1 capsid lattice.
Goldstone DC, Walker PA, Calder LJ, Coombs PJ, Kirkpatrick J, Ball NJ, Hilditch L, Yap MW, Rosenthal PB, Stoye JP, Taylor IA
Proc Natl Acad Sci U S A 111 9609-14 (2014)
PMID: 24979782

Function and Biology Details

Reactions catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124682 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; Peptidyl-prolyl cis-trans isomerase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 400 amino acids
Theoretical weight: 46.33 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: G9MAP5 (Residues: 88-304; Coverage: 46%)
  • Canonical: P00720 (Residues: 2-164; Coverage: 99%)
Gene names: E, TRIMCyp
Sequence domains:
Structure domains: Lysozyme

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2
Unit cell:
a: 124.118Å b: 59.745Å c: 146.962Å
α: 90° β: 94.77° γ: 90°
R-values:
R R work R free
0.261 0.258 0.316
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Structural principles of tumor necrosis factor superfamily signaling.
Vanamee et al. (2018)
9 more

16 mentions without citation

Restriction of HIV-1 and other retroviruses by TRIM5.
Ganser-Pornillos et al. (2019)
15 more