› 4tnt

X-ray diffraction
2.39Å resolution

Structure of the human mineralocorticoid receptor in complex with DNA

Released:
DOI: 10.2210/pdb4tnt/pdb
PDB entry 4tnt coloured by chain, front view.
PDB entry 4tnt coloured by chain, side view.
PDB entry 4tnt coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the mineralocorticoid receptor DNA binding domain in complex with DNA.
Hudson WH, Youn C, Ortlund EA
PLoS One 9 e107000 (2014)
PMID: 25188500

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118161 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Mineralocorticoid receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 103 amino acids
Theoretical weight: 11.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08235 (Residues: 593-671; Coverage: 8%)
Gene names: MCR, MLR, NR3C2
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*CP*AP*GP*AP*AP*CP*AP*CP*TP*CP*TP*GP*TP*TP*CP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 17 nucleotides
Theoretical weight: 5.16 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*AP*GP*AP*AP*CP*AP*GP*AP*GP*TP*GP*TP*TP*CP*TP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 17 nucleotides
Theoretical weight: 5.25 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 74.09Å b: 115.136Å c: 81.393Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.209 0.258
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Dynamics of ACTH and Cortisol Secretion and Implications for Disease.
Lightman et al. (2020)
5 more

3 mentions without citation

Crystal structure of the mineralocorticoid receptor DNA binding domain in complex with DNA.
Hudson et al. (2014)
2 more