› 4tpg

X-ray diffraction
3.91Å resolution

Selectivity mechanism of a bacterial homologue of the human drug peptide transporters PepT1 and PepT2

Released:
DOI: 10.2210/pdb4tpg/pdb
PDB entry 4tpg coloured by chain, front view.
PDB entry 4tpg coloured by chain, side view.
PDB entry 4tpg coloured by chain, top view.
Source organisms:
Primary publication:
Selectivity mechanism of a bacterial homolog of the human drug-peptide transporters PepT1 and PepT2.
Guettou F, Quistgaard EM, Raba M, Moberg P, Löw C, Nordlund P
Nat Struct Mol Biol 21 728-31 (2014)
PMID: 25064511

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-165190 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proton:oligopeptide symporter POT family Chains: A, B
Molecule details ›
Chains: A, B
Length: 523 amino acids
Theoretical weight: 57.22 KDa
Source organism: Shewanella oneidensis MR-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8EHE6 (Residues: 1-516; Coverage: 100%)
Gene name: SO_1277
Sequence domains: POT family
Structure domains: MFS general substrate transporter like domains
Ala-L-3-Br-Tyr-Ala Chain: E
Molecule details ›
Chain: E
Length: 3 amino acids
Theoretical weight: 481 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand LMT 2 x LMT
1 modified residue:
Ligand DBY 1 x DBY

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 82.89Å b: 108.09Å c: 206.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.262 0.261 0.29
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
6 more

4 mentions without citation

Recent advances in understanding prodrug transport through the SLC15 family of proton-coupled transporters.
Minhas et al. (2020)
3 more