› 4tpj

X-ray diffraction
3.2Å resolution

Selectivity mechanism of a bacterial homologue of the human drug peptide transporters PepT1 and PepT2

Released:
DOI: 10.2210/pdb4tpj/pdb
PDB entry 4tpj coloured by chain, front view.
PDB entry 4tpj coloured by chain, side view.
PDB entry 4tpj coloured by chain, top view.
Source organism: Shewanella oneidensis MR-1
Primary publication:
Selectivity mechanism of a bacterial homolog of the human drug-peptide transporters PepT1 and PepT2.
Guettou F, Quistgaard EM, Raba M, Moberg P, Löw C, Nordlund P
Nat Struct Mol Biol 21 728-31 (2014)
PMID: 25064511

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165191 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proton:oligopeptide symporter POT family Chains: A, B
Molecule details ›
Chains: A, B
Length: 523 amino acids
Theoretical weight: 57.22 KDa
Source organism: Shewanella oneidensis MR-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8EHE6 (Residues: 1-516; Coverage: 100%)
Gene name: SO_1277
Sequence domains: POT family
Structure domains: MFS general substrate transporter like domains
ALA-ALA-ALA Chains: C, E
Molecule details ›
Chains: C, E
Length: 3 amino acids
Theoretical weight: 231 Da
Source organism: Shewanella oneidensis MR-1
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand LMT 3 x LMT
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 78.99Å b: 107.56Å c: 203.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.244 0.29
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
5 more

6 mentions without citation

Recent advances in understanding proton coupled peptide transport via the POT family.
Newstead S. (2017)
5 more