› 4tps

X-ray diffraction
1.65Å resolution

Sporulation Inhibitor of DNA Replication, SirA, in complex with Domain I of DnaA

Released:
DOI: 10.2210/pdb4tps/pdb
PDB entry 4tps coloured by chain, front view.
PDB entry 4tps coloured by chain, side view.
PDB entry 4tps coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Structure and interactions of the Bacillus subtilis sporulation inhibitor of DNA replication, SirA, with domain I of DnaA.
Jameson KH, Rostami N, Fogg MJ, Turkenburg JP, Grahl A, Murray H, Wilkinson AJ
Mol Microbiol 93 975-91 (2014)
PMID: 25041308

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138724 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sporulation inhibitor of replication protein SirA Chains: A, C
Molecule details ›
Chains: A, C
Length: 154 amino acids
Theoretical weight: 18.72 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P45707 (Residues: 1-148; Coverage: 100%)
Gene names: BSU17900, sirA, yneE, yoxF
Sequence domains: Sporulation inhibitor of replication protein SirA
Structure domains: Sporulation inhibitor of replication protein SirA
Chromosomal replication initiator protein DnaA Chains: B, D
Molecule details ›
Chains: B, D
Length: 85 amino acids
Theoretical weight: 9.74 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05648 (Residues: 1-82; Coverage: 18%)
Gene names: BSU00010, dnaA, dnaH
Sequence domains: DnaA N-terminal domain
Structure domains: GMP Synthetase; Chain A, domain 3

Ligands and Environments

2 bound ligands:
Ligand BME 2 x BME
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 77.287Å b: 34.685Å c: 84.735Å
α: 90° β: 102.09° γ: 90°
R-values:
R R work R free
0.13 0.126 0.193
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Replication Initiation in Bacteria.
Chodavarapu et al. (2016)
2 more

12 mentions without citation

Control of Initiation of DNA Replication in Bacillus subtilis and Escherichia coli.
Jameson et al. (2017)
11 more