› 4tq2

X-ray diffraction
1.95Å resolution

Structure of S-type Phycobiliprotein Lyase CPES from Guillardia theta

Released:
DOI: 10.2210/pdb4tq2/pdb
PDB entry 4tq2 coloured by chain, front view.
PDB entry 4tq2 coloured by chain, side view.
PDB entry 4tq2 coloured by chain, top view.
Source organism: Guillardia theta CCMP2712
Primary publication:
Insights into the biosynthesis and assembly of cryptophycean phycobiliproteins.
Overkamp KE, Gasper R, Kock K, Herrmann C, Hofmann E, Frankenberg-Dinkel N
J Biol Chem 289 26691-26707 (2014)
PMID: 25096577

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125793 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative phycoerythrin lyase Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 21.66 KDa
Source organism: Guillardia theta CCMP2712
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: L1JFC7 (Residues: 80-253; Coverage: 76%)
Gene name: GUITHDRAFT_191403
Sequence domains: CpeS-like protein
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand HEZ 1 x HEZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 80.83Å b: 116.58Å c: 58.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Recent advances in production, purification and applications of phycobiliproteins.
Sonani et al. (2016)
1 more

4 mentions without citation

Insights into the biosynthesis and assembly of cryptophycean phycobiliproteins.
Overkamp et al. (2014)
3 more