› 4tqk

X-ray diffraction
2.1Å resolution

Structural basis of specific recognition of non-reducing terminal N-acetylglucosamine by an Agrocybe aegerita lection

Released:
DOI: 10.2210/pdb4tqk/pdb
PDB entry 4tqk coloured by chain, front view.
PDB entry 4tqk coloured by chain, side view.
PDB entry 4tqk coloured by chain, top view.
Source organism: Cyclocybe aegerita
Primary publication:
Structural Basis of Specific Recognition of Non-Reducing Terminal N-Acetylglucosamine by an Agrocybe aegerita Lectin.
Ren XM, Li DF, Jiang S, Lan XQ, Hu Y, Sun H, Wang DC
PLoS One 10 e0129608 (2015)
PMID: 26114302

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124800 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lectin 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 406 amino acids
Theoretical weight: 43.08 KDa
Source organism: Cyclocybe aegerita
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: H6CS64 (Residues: 2-407; Coverage: 100%)
Gene name: aal-2
Sequence domains: FG-GAP-like repeat

Ligands and Environments

1 bound ligand:
Ligand NAG 11 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 1W2B
Spacegroup: P212121
Unit cell:
a: 52.56Å b: 111.24Å c: 134.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.171 0.241
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

5 citation in other articles

Recombinant fungal lectin as a new tool to investigate O-GlcNAcylation processes.
Machon et al. (2017)
4 more

1 mention without citation

Structural Basis of Specific Recognition of Non-Reducing Terminal N-Acetylglucosamine by an Agrocybe aegerita Lectin.
Ren et al. (2015)