› 4tr6

X-ray diffraction
1.5Å resolution

Crystal structure of DNA polymerase sliding clamp from Bacillus subtilis

Released:
DOI: 10.2210/pdb4tr6/pdb
Model geometry
Fit model/data
PDB entry 4tr6 coloured by chain, front view.
PDB entry 4tr6 coloured by chain, side view.
PDB entry 4tr6 coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Differential modes of peptide binding onto replicative sliding clamps from various bacterial origins.
Wolff P, Amal I, Oliéric V, Chaloin O, Gygli G, Ennifar E, Lorber B, Guichard G, Wagner J, Dejaegere A, Burnouf DY
J Med Chem 57 7565-76 (2014)
PMID: 25170813

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138726 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta sliding clamp Chains: A, B
Molecule details ›
Chains: A, B
Length: 380 amino acids
Theoretical weight: 42.37 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P05649 (Residues: 1-378; Coverage: 100%)
Gene names: BSU00020, dnaG, dnaN
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P1
Unit cell:
a: 43.22Å b: 68.33Å c: 85.31Å
α: 65.77° β: 82.84° γ: 86.79°
R-values:
R R work R free
0.178 0.176 0.204
Expression system: Escherichia coli

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Citations

4 review citations

Recent advancements in the development of anti-tuberculosis drugs.
Chetty et al. (2017)
3 more

5 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
4 more