› 4tsy

X-ray diffraction
3.14Å resolution

Crystal structure of FraC with lipids

Released:
DOI: 10.2210/pdb4tsy/pdb
PDB entry 4tsy coloured by chain, front view.
PDB entry 4tsy coloured by chain, side view.
PDB entry 4tsy coloured by chain, top view.
Source organism: Actinia fragacea
Primary publication:
Structural basis for self-assembly of a cytolytic pore lined by protein and lipid.
Tanaka K, Caaveiro JM, Morante K, González-Mañas JM, Tsumoto K
Nat Commun 6 6337 (2015)
PMID: 25716479

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110921 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DELTA-actitoxin-Afr1a Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 179 amino acids
Theoretical weight: 19.75 KDa
Source organism: Actinia fragacea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B9W5G6 (Residues: 1-179; Coverage: 100%)
Sequence domains: Sea anemone cytotoxic protein
Structure domains: Cytolysin/lectin

Ligands and Environments

3 bound ligands:
Ligand HXJ 12 x HXJ
Ligand SO4 19 x SO4
Ligand HTO 1 x HTO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: C2221
Unit cell:
a: 151.337Å b: 199.879Å c: 120.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

25 review citations

Pore-forming toxins: ancient, but never really out of fashion.
Dal Peraro et al. (2016)
24 more

30 mentions without citation

Assembling the puzzle: Oligomerization of α-pore forming proteins in membranes.
Cosentino et al. (2016)
29 more